Abstract
Aims: to investigate serum biochemical changes (malondialdehyde levels and T-AOC) and the histopathological changes of salivary glands. Materials and Methods: Twelve healthy mature male rabbits were included in the study.Group one (6 animals): the rabbits were maintained on a standard diet and water without any treatment for 30 days. Group two (6 animals): the rabbits were given) NaF at the dose of 20 mg/kg/day (dissolved in 400 ml of distilled water for 30 days. At the end of one month of treatment, the animals were sacrificed and (5ml) of blood samples were taken from all animals via jugular vein during the animal sacrificing. Serum were separated from all samples and stored at -20 ˚C till time of analysis by colorimetric Assay kit for both total antioxidant capacity (T-AOC) by (Elabscience, Cat. No. E-BC-K136-S, TBA method) and malondialdehyde (MDA) by (Elabscience, Cat.No.E-BC-K025-S, TBA method).Tissue samples from parotid and sub-mandibular salivary glands were isolated and examined under a light microscope for histopathological changes. Results: Significant differences were founded between the values of serum biochemical markers between control and treatment groups. T-AOC in control group was higher than in treatment group, while MDA level in treatment group was higher than control group. Sections of the parotid and submandibular glands taken from the control group appeared to have the normal histological structure compared to the treated group, which showed an acute inflammatory cell infiltration, Hyperemia with thickening of blood vessels wall. Conclusions: NaF at dose of 20 mg/kg/day for 30 days can lead to a decrease in T-AOC with considerable inflammatory changes in tissues of salivary glands of rabbits.
