Abstract
The adherence of Candida albicans on acrylic resin denture base surface is co-nsidered as the first step in the pathogeni-city of candida–associated denture stoma-titis which is the most prevalent form of oral candidiasis in denture wearers. This study was an in vitro study aimed to eval-uate the anifungal effect of fluconazole, nystatin and chlorhexidine and their anti-adherent effect on Candida albicans cells that attached on the fitting surface of den-ture base. It was determined, using broth microdilution method at two concentra-tions for each drug, and the results were determined using spectrophotometer. All drugs had a highly significant antifungal effect on Candida albicans isolate at their two concentrations. The effect of saliva on the adherence of Candida albicans on the fitting surface of denture base was also considered. This study concluded the importance of saliva in increasing the microbial adherence; i.e., the mean number of adherent cells on ac-rylic resin samples in the absence of saliva was 7.4 + 1.9 cell/mm2 and with the pre-sence of saliva the number was 29.1 + 2.7 cells/mm2. The number of remaining adh-erent cells was counted using fluortescent microscope. The mean number of remain-ing cells which adhered on acrylic resin samples after the immersion in first con-centration fluconazole, nystatin and chlorhexidine for one hour respectively were 4, 5 and 2.6 cells/mm2 and for the second concentration/1 hour the numbers were 3.2, 4.1 and 1.7 cells/mm2 compared to the normal number of adherent cell 29.1 + 2.7 at p<0.01.
