Abstract
Aims:_x0001_ Treponema denticola is one of the most important
periodontal pathogens because of its high lytic enzymatic
activity leading to tooth lose and its ability to invade tissues
and spread via blood stream causing systemic infections, this
study coming to be the first local study concerning with this
anaerobic fastidious bacterium and looking at the possibility
of rely on the culturing methods in determining the most
preferred chemotherapeutic drugs for limiting of bacterial
activity and spread, as well as investigating the range of the
antimicrobial activity of some mouth rinses and number of
antibiotics._x0001_ Materials and Methods: 139 samples were
collected from periodontal pockets with a depth of _x0001_3 mm
under supervision of specialist dentitist, then placed in a
reduced transport medium. The activity and effective
spectrum of different concentrations for three kinds of widely
used mouth rinses in the treatment of periodontal infections
including Biofresh K, Biofresh F and Zak towards this
bacterium was studied using sensitivity test methods,
furthermore, the minimum inhibitory cocentration of
antibiotics amoxillin, ciprofloxacin, clindamycin,
azithromycin and mitronidazole was determined using
antibiotic discs diffusion method. Results: The results
showed that Biofresh K is the best in the term of bacterial
killing as its inhibition activity continued to 1:16 dilution and
when the sensitivity of T.denticola isolates to some
antibiotics was tested, it was appeared that ciprofloxacin is the
best causing growth inhibition with the lowest minimum
inhibitory cocentration (0.0001mg), and when the synergistic
effect of the tested antibiotics was studied it is becoming clear
that the lowest antibiotic concentrations can cause growth
inhibition when the two antibiotics (mitronidazole +
ciprofloxacin) or (mitronidazole + amoxillin) are used in
combination. Conclusions: It is possible to depend on
culturing methods for determining the sensitivity of the
bacterium T.denticola to chemotherapeutic drugs. Biofresh K
is the best among the rinses under study and the antibiotic CIP
is the best one with the lowest MIC and the lowest
concentration of the antibiotics resulting in growth inhibition
can be achieved when they are used in combination.
