Abstract
Aims: The study aims to evaluate the influence of various denture cleansers on colonization of Candi-da albicans to Cobalt–Chromium alloy denture base and the subsequent roughness assosiated with these cleansers. Materials and methods: Thirty–six samples of Co–Cr denture base and five cleansers, four prepared(alum, salt, soda+vinegar, soda+thymol), one commercial ( protefix ) and distilled wa-ter(D.W) as a control were used. Samples were immersed in denture cleansers for one month in which each cleanser had 6 samples excluding 6 samples in D.W as a control. Half of samples for each cleans-er were immersed ½ hr per day and the other half immersed 8hrs per day through one month, before microbiological examination, samples were tested for surface roughness using profilometer. Candida albicans cell suspension was incubated with the test samples for 1hr at 37°C after which the test sam-ples were immersed in their cleansers for 1hr. Visualization, inspection and enumeration of adherent C.albicans cells and detection of the anti–adherent effect of the cleansers was achieved by using light microscopy. Results: The results demonstrated insignificant difference in surface roughness of Co–Cr alloy denture base in the cleansers at 1/2 hr and a significant difference at 8 hrs immersion. There was a significant difference in C.albicans colonization to Co–Cr denture base in which all the cleansers showed less adhesion than control. The results also revealed that (soda+Thymol oil) cleanser ex-pressed the least values of colonization and roughness among other cleansers. Conclusions: The cleansers were effective as anti–adherent yeast cells to Co–Cr denture base and showed roughness de-grees less than control
