is to evaluate the effect of three different disinfectant solutions on shear bond strength of artificial acrylic teeth for retention to flexible denture base material at three different periods of time. Materials and Methods: The total number of samples was (60) divided into 4 groups according to the three chemical disinfectant solution which used and the control distilled water group: Group( I) vinegar group, Group( II) salt group , Group(III) Chlorohexidine group , Group (IV ) the control distilled water group, where each group was subdivided into three subgroups according to disinfection periods which are 1week, 1month and 3month where each group contain five samples. The samples of shear bond strength test were consist of acrylic teeth with T-shape design for retention attached to rod of valplast The test was performed using a Universal testing machine. ANOVA, DuncunP,Ps multiple range and Post Hoc Tests were carried out to determine the significant difference at p ≤ 0.01%. Results: The results appeared that control distilled water group has the highest value for the three periods of disinfection (1 week,1month,3months), while vinegar solution group has the least value. The results showed that there were significant differences between the four tested groups and also there were significant differences among all periods of time of disinfections. Conclusion: There was a significant difference of shear bond strength between artificial teeth and flexible denture base material after disinfection in the four disinfectant solutions for all periods of time of immersion. Vinegar group had highest effect on the bond strength while the distilled water group had the least effect after all periods of time of disinfections. Shear bond strength decreased with increasing the time immersion. The shear bond strength was lowest in chloherxidine group as the time of immersion increase when compared with the two other groups of disinfection solutions in this study.