The aim of this study was to evaluate the antimicrobial
effect of 10% water extraction of Salvadora persica (Miswak)
when used clinically as an endodontic irrigant.
Twenty four uniradicular teeth with necrotic pulps were
chosen. The patients were divided randomly into 2 groups:
Experimental group, in which water extract of Salvadora
persica (10%) was used as a root canal irrigant; and control
group, in which distilled water was used as a root canal irrigant.
Bacteriological samples were obtained from the canal at
the step of working length determination (before the canal
was subjected to instrumentation and irrigation procedures),
and at the end of the biomechanical instrumentation procedures
by using a sterile K–file. The file was separated from the
handle using a sterile wire cutter, and the severed portion was
placed in a sterile screw–capped vial containing 5 ml of thioglycollate
broth as a transport media. A 0.1 ml of thioglycollate
broth was inoculated on each of two brain–heart infusion
agar plates: One plate was incubated under aerobic conditions,
and the other was incubated under anaerobic conditions
using anaerobic jar and gas pack anaerobic system. Both plates
were incubated at 37 ºC for 24 hours; then, the number of
bacterial colonies was counted.
The results revealed that 10% water extraction of Salvadora
persica is an effective antimicrobial agent when utilized
clinically as an irrigant in the endodontic treatment of teeth
with necrotic pulps.