Aims: the present study aimed to analyze the effects of the incorporation of Zinc Oxide nano-fillers (ZnO NPs) with size (10-30 nm) at one concentration (1%) on color change and porosity and FTIR compared to milled CAD/CAM and conventional denture base materials. Materials and methods: the number of samples were 53 which divided into twenty samples for color change test (control, 1 % ZnO NPs), thirty samples for porosity test and three specimens for FTIR test for (control, 1 % ZnO NPs,CAD/CAM ) . Results: the results demonstrated that there was a significant change in color, with comparable FTIR spectra for the PMMA-ZnO nanocomposite at ZnO nanoparticles (1%), when compared to control and non-significant reduction to porosity test. CAD/CAM denture base showed significant low mean value as compared between control and (1%) zinc oxide group and also showed similar with non- significant difference spectra on FTIR test as compared to the control group. Conclusions: there is unacceptable color change in addition that use of ZnO nanoparticles as dental fillers at 1% by weight of PMMA denture base material and no chemical interaction between zno nanoparticles and CAD/CAM PMMA denture base which has superiority in porosity test.