Aims: The objective of this study was to compare the histological wound healing potential between black silk suture and bioadhesive glue after induced surgical skin incisions. Materials and methods: The study was conducted on (18) male New Zealand rabbits, randomly divided into two groups (9 animals per group) according to the healing periods (1st,3rd, and 7th) days. Two incisions were made on the back skin of each rabbit bilaterally. In the first group, the defect was sutured with black silk suture, and adhesive glue Derma + Flex for the second group applied post operatively. Three rabbits were randomly selected from each group at the (1st,3rd, 7th) days, and biopsies were taken. The biopsy specimens were subjected to histological assessment to assess the healing parameters of the primary wound healing process. Results: The results of inflammatory cells infiltration grading showed that in day 1 group II and day 3 group I was the highest and at day 7 was the lowest in both groups. For granulation tissue formation, the results showed that at day 1 and day 3 was the same but high in group II day 7. Re-epithelization scoring was highest in group II day 3 and day 7. According to the time period, there were no significant differences in group versus group (within the same day). A very close activity of the two methods of wound closure in relation to their use in primary wounds in skin was noticed. Conclusions: The use of bioadhesive Derma + Flex® glue for closure of primary wounds in skin gives a satisfying result, for better healing and isolation to avoid infection in the appropriate time with less equipment’s in comparison with black silk suture.