Aims: The objective of this study was to compare wound healing potential between black silk suture and bioadhesive glue after induced surgical intraoral mucosal incisions in rabbits and assessed histologically. Materials and methods: The study was conducted on (18) male New Zealand rabbits, randomly divided into two groups (9 animals per group) according to the healing periods (1st,3rd, and 7th) days. Two incisions were made on the buccal mucosa of each rabbit bilaterally. For the first group, the defect was sutured with black silk suture, and adhesive glue Derma + Flex for the second group applied post operatively. Three rabbits were randomly selected from each group at the (1st,3rd, 7th) days, and biopsies were taken. The biopsy specimens were subjected to histological assessment to assess the healing parameters of the primary wound healing process. Results: Inflammatory cell infiltration grading in day 1and day 3 group I was the highest and on day 7 was the lowest in both groups. Granulation tissue formation in day 1 and day 3 was the same but high in group II day 7. Re-epithelization scoring was highest at day 7 for both groups. According to the time period, there were no significant differences in group versus group (within the same day). Conclusions: Use of bioadhesive Derma+Flex® glue for closure of primary wounds gave a satisfied result for better healing and isolation to avoid of infection in appropriate time with less equipment’s in comparison with black silk suture.