The Antibacterial Effect of Magnetized Water on Oral Microorganisms

Aims: The purpose of this study was to investigate the effect of magnetized water as an oral irrigant. Materials and Methods: Samples of magnetized water were prepared using a locally made device at different magnetic strengths; 500, 850, 1500 and 1900 Gauss; four groups of healthy individuals with certain criteria (25 for each group) rinsed their mouth with 10 ml of the magnetized water and two saliva samples; before and after irrigation were collected, transferred to the laboratory for colony counting. Results: significant differences were found between the CFU/ml of oral bacteria from baseline saliva samples and after irrigating with magnetized water with different magnet strengths at p ≤ 0.005. Conclusion : As the good antibacterial, fresh, cheap , and available; the regular use of magnetized water was suggested as the irrigant solution for good oral hygiene.


INTRODUCTION
Magnetic water is based on science that started to invade many areas of the worlds, some consider it a magic description that achieves many benefits at one time. In industry, it achieves a real fence of equipment and production lines against wear and tear and rapidly deteriorating salts. Magnetism is well known in the field of physics. Magnets prove to The Antibacterial Effect of Magnetized Water on Oral Microorganisms be a strong safeguard against illness and serve as a highly beneficial preventive device. When water passes through the magnetic field, it undergoes certain changes. The magnetic field alters the electrical characteristics of hydrogen ions as well as minerals (1) . It is also known to draw pain out from the body, reduces stiffness of joints and muscles, and removes a toothache immediately. When water passes through the magnetic field, it undergoes certain changes.
The magnetic field alters the electrical characteristics of hydrogen ions as well as minerals. The bio-south magnets help in reducing scale buildup. The bio-north magnetic water is used in the treatment of various diseases. The bio-south magnetic field stimulates the system (2) The force of magnetism influences the life of organisms. When a permanent magnet is kept in contact with water continuously for known time, the magnetic flux of magnet influences the water and becomes magnetized, also acquires magnetic properties. Best results are obtained if the water is used immediately after treatment (3). Its acceptability and longterm use as the mouth rinse by patients is associated with these side effects, more appropriate alternatives, especially for children, is needed. Thus, there has been a focus to find another natural alternative and cheap agents.
These natural products have major advantages, that no side effect has been reported after their use (4)

MATERIALS AND METHODS a-Magnetized Water sample preparation:
The tap water was magnetized by a locally made device at different magnetic strengths; where a Pyrex-type laboratory glass tube (

c-Microbiological method:
One milliliter of each sample (vial) was diluted decimally and then one milliliter of each dilution was cultured on Brain Heart Infusion agar plates (three replicates for each dilution), all petri dishes were incubated in 37 C 0 for 24-48 hours, and the number of colonies was counted as CFU/ml (10) .

d-Statistical method:
The mean of the three replicates of the CFU/ml for saliva samples before and after rinsing with magnetized water was analyzed and compared using the SPSS program V.25 . The means for groups between homogeneous subsets were displayed, compared by Wilcoxon Signed Rank Test. The differences were considered significant at p ≤ 0.005.

RESULTS
After incubation, the bacterial cultures

DISCUSSION
Human body is composed of over 70% water. Circulation, absorption, digestion, and excretion depend on water that is required for blood, lymph, skin, and muscles. The pH of the human body is more acidic when we are sick. Magnetized water is alkaline, which raises our body pH, which allows the body to remove the toxins. Bio-magnetized water is energybuilding, activating, and detoxifying (2,11) The relationship between magnetic power and water, what we understand from the old literature is that water -H 2 O, with its bonds of hydrogen is related to each other, and these linkages may be bilateral or multiple. This may reach a large number of bonds, if we place water molecules within a magnetic field, these bonds between either atoms change or dissolve, and the dissociation absorb energy and reduces the water atoms union level, raising the ability of electrolysis and affecting the crystal dissolution .
Simply, water treatment with magnetic fields brings back the balance and natural energy that nature intended. Magnetized water has hydroxyl (OH-) ions much more than tap water, which makes it alkaline and reduces the acidity. Tap water has a pH of about 7. While magnetized water is alkaline, its pH is as high as 9.2. One benefit of the magnetic force is reducing the surface tension of the water, making it feel softer, wetter, thinner, and more absorbable, and is able to penetrate cell walls carrying the nutrients (9). Controversy regarding the impact effects of the magnetic field on water results from the physical structure of liquid water. Hydrogen micro clusters are formed when water is magnetized, these clusters react with oxygen molecules forming sodium hydroxide (NaOH), the water's pH is increased making it extra alkaline (13 ( The oral irrigator using magnetized water could be a beneficial adjunct to prevent the accumulation of calculus in periodontal patients, but it has a minimal effect on plaque reduction. Also, there is clinical improvement in the gingival index, but no statistical significance found (14). It is shown that treatment with magnetized water highly reduced the calculus accompanied or coexisted with caries (14) . Magnetized water prevents the bonding process between the plaque and the teeth by -magneto-hydro-dynamics‖, which prevents naturally occurring mineral deposits in fluids (4)