Print ISSN: 1812-1217

Online ISSN: 1998-0345

Keywords : antibacterial

antibacterial activity of the ethanolic extract of some plant leaves on aerobic and anaerobic pathogens

Ghada Y Abdul- Rahman

Al-Rafidain Dental Journal, 2011, Volume 11, Issue 3, Pages 215-219
DOI: 10.33899/rden.2011.164455

Aims: To evaluate the antibacterial effect of ethanolic extracts
of three medicinal plants (Camellia sinensis,green and black
tea, and Ziziphus spp) on different aerobic and anaerobic
bacteria. Material and Methods: The leaves of black tea,
green tea, and ziziphus from local markets were collected and
extracted in ethanol, the antibacterial activity of 50 ,5, and
0.5% of the ethanol extracts against Escherechia coli,
Staphylococcus aureus, Actinobacilus
actinomycetemcomitans, viridians streptococci and black
pigmenting bacteria were investigated using paper disk
diffusion technique. Results: The results obtained showed that
ethanolic extract exhibited antibacterial activities against
Escherechia coli, Staphylococcus aureus, Actinobacilus
actinomycetemcomitans, viridians streptococci and black
pigmenting bacteria. The extracts produced inhibition zones
ranging from 2 – 32 mm against the test bacteria compared
with chlorhexidin (12-15mm). Conclusions: The ethanolic
extracts of the examined plants were effective antibacterial
agents against the aerobic and anaerobic bacteria especially
the periodontopathogens so we suggest to use them in
treatment and prevention of gingivitis and periodontitis.

Evaluation of the Antibacterial Activity of Citrus Juices: An In Vitro Study

Nahla O Tawfik; Siba M Al-Haliem; Waidulla N Al-Ani

Al-Rafidain Dental Journal, 2010, Volume 10, Issue 2, Pages 376-382
DOI: 10.33899/rden.2010.9030

Aims: This work aimed at determining the antibacterial activity of three Citrus Juice on Staphylococ-cus aureus, Proteus vulgaris and Pseudomonas aeruginosa. Materials and Methods: The antibacteri-al activities of juices of three types of Citrus (C.) fruit, C. limon (lemon), C. aurantium (bitter orange) and C. paradisi (grapefruit), against some gram positive and gram negative bacteria using four concen-trations of these juices by agar diffusion method with measurement of diameter of the zone of inhibi-tion around the extracts. Results: The results confirmed the presence of anti bacterial activity of the Citrus juice. The highest inhibition zone (18 mm) was observed with a 10% concentration of C. para-dises juice on Staphylococcus aureus, but Proteus vulgaris and Pseudomonas aeruginosa were resis-tant. C. limon and C. aurantium juice at 10% and 5% concentration gave positive results with bacteri-cidal effects on the three tested bacteria (Staphylococcus aureus, Proteus vulgaris and Pseudomonas aeruginosa). The zones of inhibition of C. limon were 10, 12 and 13mm for 5% and 15, 21 and 20 mm for 10% respectively. For C. aurantium, they were 18, 19 and 20 mm for 5% concentration and 16, 19 and 19 mm for 10% concentration respectively. Those effects were better than those produced by stan-dard antibiotic disc used for comparison. Conclusions: The use of different concentrations of Citrus juice extracts had an effective antibacterial activity against Staphylococcus aureus, Proteus vulgaris and Pseudomonas aeruginosa.

The Antibacterial Effect of Fig (Leaves Extract and Latex) on Enterococcus faeca-lis as Intracanal Medicament. (An in vi-tro study)

Nawal A AL-Sabawi

Al-Rafidain Dental Journal, 2010, Volume 10, Issue 1, Pages 62-71
DOI: 10.33899/rden.2010.8966

Aims: This study perfoemed were to evaluate the effectiveness of ethanolic extract of leaves (EEL) at different concentrations and latex (LX) of Ficus carica against Enterococcus faecalis, and to evaluate the most effective concentration of EEL and LX against Enterococcus faecalis in dentinal tubules when used as intracanal medicament. Materials and Methods:The different concentrations of EEL (5%,2.5%,1.25%,0.6%,0.3%,0.1%) and LX against Enterococcus faecalis was evaluated by broth mi-crodilution method using spectrophotometer. One hundred and twenty freshly extracted single canal humen teeth were chosen. They were section at cementoenamal junction, instrumented, sectioned apic-al one third, and then sterilized. They are then contaminated with Enterococcus faecalis solution and incubated. These samples divided into three groups randomly. Root canals filled with 5% EEL, LX, and formocrsol and maintained for one day for group one, for three days for group two, and seven days for group three. The medicaments removed from the canal and dentin chips was obtained from the can-al and weight 10 mg that will be diluted in brain heart infusion broth, and cultured on blood agar then the number of colonies recovery were estimated. Results: this study obtained that 5% EEL had best effect than other concentrations but its effect less than LX but significantly not different. Result also obtained that 5% EEL and LX capable after seven day to eliminate the Enterococcus faecalis from In-fected dentinal tubule, while formocresol can not. Conclusions: The EEL at 5% and LX had sufficient antibacterial effect against Enterococcus faecalis in the infected dentinal tubules when they are used as intracanal medicaments.